2024
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Modulating the immunodominance hierarchy of immunoglobulin germline-encoded structural motifs targeting the influenza hemagglutinin stem
Ataca S, Sangesland M, de Paiva Fróes Rocha R, Torrents de la Peña A, Ronsard L, Boyoglu-Barnum S, Gillespie RA, Tsybovsky Y, Stephens T, Moin SM, Lederhofer J, Creanga A, Andrews SF, Barnes RM, Rohrer D, Lonberg N, Graham BS, Ward AB, Lingwood D, Kanekiyo M. Modulating the immunodominance hierarchy of immunoglobulin germline-encoded structural motifs targeting the influenza hemagglutinin stem. Cell Rep. 2024 Nov 22; 43(12):114990.
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Eliciting a single amino acid change by vaccination generates antibody protection against group 1 and group 2 influenza A viruses
Ray R, Nait Mohamed FA, Maurer D P., Huang J, Alpay B A., Ronsard L, Xie Z, Han J, Fernandez-Quintero M, Anh Phan Q, Ursin R L., Vu M, Kirsch K H., Prum T, Rosado V C., Bracamonte-Moreno T, Okonkwo V, Bals J, McCarthy C, Nair U, Kanekiyo M, Ward A B., Schmidt A G., Batista F D., Lingwood D.
Eliciting a single amino acid change by vaccination generates antibody protection against group 1 and group 2 influenza A viruses. Immunity, 2024 57:1141–1159. -
A VRC13-like bNAb response is associated with complex escape pathways in HIV-1 envelope
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An approach for Antigen-Agnostic Identification of Virus-Like Particle-Displayed Epitopes that Engage Specific Antibody V Gene Regions
Peabody J, Core SB, Ronsard L, Lingwood D. Peabody DS, Chackerian B. 2024. An approach for Antigen-Agnostic Identification of Virus-Like Particle-Displayed Epitopes that Engage Specific Antibody V Gene Regions. Methods in Molecular Biology 2720:55-74. PMID: 37775657
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Distinct Functional Humoral Immune Responses Are Induced after Live Attenuated and Inactivated Seasonal Influenza Vaccination
Tong X, Deng Y, Cizmeci D, Fontana L, Carlock MA, Hanley HB, McNamara RP, Lingwood D, Ross TM, Alter G. 2024. Distinct Functional Humoral Immune Responses Are Induced after Live Attenuated and Inactivated Seasonal Influenza Vaccination. Journal of Immunology 212:24-34. PMID: 37975667
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Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds
Wamhoff EC1, Ronsard L1, Feldman J, Knappe GA, Hauser BM, Romanov A, Case JB, Lam E, St Denis K, Boucau J, Barczak AK, Balazs AB, Diamond MS, Schmidt AG*, Lingwood D*, Bathe M*. 2024. Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds. Nature Communications 15, 795. PMID: 38291019. 1equal contribution; *co-corresponding
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Heterologous sarbecovirus receptor binding domains as scaffolds for SARS-CoV-2 receptor binding motif presentation
Hauser BM, Sangesland M, Lam EC, Denis KJS, Sheehan ML, Vu ML, Cheng AH, Balazs AB, Lingwood D, Schmidt AG. 2024. Heterologous sarbecovirus receptor binding domains as scaffolds for SARS-CoV-2 receptor binding motif presentation. ACS Infectious Diseases doi: 10.1021/acsinfecdis.3c00483PMID: 38281136
2023
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Engaging an HIV vaccine target through the acquisition of low B cell affinity
Ronsard L., Yousif AS, Nait Mohamed FA, Feldman J, Okonkwo V, McCarthy C, Schnabel J, Caradonna T, Barnes RM, Rohrer D., Lonberg N, Schmidt A, and Lingwood D 2023. Engaging an HIV vaccine target through the acquisition of low B cell affinity. Nature Communications 14:5249. PMID: 37640732
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Antibody-mediated NK cell activation as a correlate of immunity against influenza infection
Boudreau C, Burke J, Yousif AS, Sangesland M, Jastrzebski S, Verschoor C, Kuchel G, Lingwood D, Kleanthous H, de Bruijn I, Landolfi V, Sridhar S, and Alter G. 2023. Antibody-mediated NK cell activation as a correlate of immunity against influenza infection. Nature Communications 14:5170. PMID: 37620306
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Pre-existing Fc profiles shape the evolution of neutralizing antibody breadth following influenza vaccination
Boudreau CM, Burke JS 4th, Roederer AL, Gorman MJ, Mundle S, Lingwood D, Delagrave S, Sridhar S, Ross TM, Kleanthous H, Alter G. Cell Reports Medicine. 2023, 4: 100975. PMID: 36921600.
2022
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Engineered cell entry links receptor biology with single-cell genomics
Yu, B, Shi Q, Belk JA, Yost KE, Parker KR, Li R, Liu BB, Huang H, Lingwood D. Greenleaf WJ, Davis MM, Satpathy AT, Chang HY. 2022. Systematic discovery of receptor-ligand biology by engineered cell entry and single-cell genomics. Cell, 2022. 185:4904-4920. PMID: 36516854.
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Cross-reactive SARS-CoV-2 epitope targeted across donors informs immunogen design
Hauser BM, Feldman J, Sangesland M, Ronsard L, St Denis KJ, Sheehan ML, Cao Y, Boucau J, Windsor IW, Cheng AH, Vu ML, Cardoso MR, Kannegieter T, Balazs AB, Lingwood D, Garcia-Beltran WF, and Schmidt AG. 2022. Cross-reactive SARS-CoV-2 epitope targeted across donors informs immunogen design. Cell Reports Medicine 3:100834. PMID: 36423634
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An epitope-enriched immunogen expands responses to a conserved viral site
Caradonna TM, Ronsard L, Yousif A, Windsor IW, Hecht R, Bracamonte-Moreno T, Roffler AA, Maron MJ, Feldman J, Marchiori E, Barnes RM, Rohrer D, Lonberg N, Oguin TH, Sempowski GD, Kepler TB, Kuraoka M, Lingwood D*, and Schmidt AG*. Cell Reports.2022 41:111628. PMID: 36351401. *co-corresponding
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Allelic polymorphism controls autoreactivity and vaccine elicitation of human broadly neutralizing antibodies against influenza virus
Sangesland M, Torrents de la Peña A, Boyoglu-Barnum S, Ronsard L, Nait Mohamed FA, Bracamonte Moreno M, Barnes RM, Rohrer D, Lonberg N, Ghebremichael M, Kanekiyo M, Ward A, and Lingwood D. Immunity. 2022 55:1693-1709 PMID:35952670.
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Humoral responses to the SARS-CoV-2 spike and receptor binding domain in context of pre-existing immunity confer broad sarbecovirus neutralization
Hauser BM, Sangesland M, Lam EC, Feldman J, Balazs AB, Lingwood D and Schmidt AG. Frontiers in Immunology. 2022 13:902260. PMID: 35990628.
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Antibodies from primary humoral responses modulate recruitment of naive B cells during secondary responses
, , , , , , , Wang X, Cottrell CA, , , , , Groshel B, , , , Phelps N, Adachi Y, Eskandarzadeh S, Kubitz M, Burton DR, Lingwood D, Schmidt AG, Nair U, Ward AB, Schief WR, Batista FD. Immunity. 2022 55:1856-1871. PMID: 35987201.
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Antigen identification and high-throughput interaction mapping by reprogramming viral entry.
Dobson CS, Reich AN, Gaglione S, Smith BE, Kim EJ, Dong J, Ronsard L, Okonkwo V, Lingwood D, Dougan M, Dougan SK, Birnbaum ME. Nat Methods. 2022, 19:449-460. PMID: 35396484
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Rationally designed immunogens enable immune focusing following SARS-CoV-2 spike imprinting.
Hauser BM, Sangesland M, St Denis KJ, Lam EC, Case JB, Windsor IW, Feldman J, Caradonna TM, Kannegieter T, Diamond MS, Balazs AB, Lingwood D, Schmidt AG. Cell Rep. 2022, 38:110561. PMID: 35303475
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Mannose-binding lectin and complement mediate follicular localization and enhanced immunogenicity of diverse protein nanoparticle immunogens.
Read BJ, Won L, Kraft JC, Sappington I, Aung A, Wu S, Bals J, Chen C, Lee KK, Lingwood D, King NP, Irvine DJ. Cell Rep. 2022, 38:110217. PMID: 35021101
2021
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Public Immunity: Evolutionary Spandrels for Pathway-Amplifying Protective Antibodies.
Sangesland M, Lingwood D. Front Immunol. 2021, 12:708882. PMID: 34956170
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A particulate saponin/TLR agonist vaccine adjuvant alters lymph flow and modulates adaptive immunity.
Silva M, Kato Y, Melo MB, Phung I, Freeman BL, Li Z, Roh K, Van Wijnbergen JW, Watkins H, Enemuo CA, Hartwell BL, Chang JYH, Xiao S, Rodrigues KA, Cirelli KM, Li N, Haupt S, Aung A, Cossette B, Abraham W, Kataria S, Bastidas R, Bhiman J, Linde C, Bloom NI, Groschel B, Georgeson E, Phelps N, Thomas A, Bals J, Carnathan DG, Lingwood D, Burton DR, Alter G, Padera TP, Belcher AM, Schief WR, Silvestri G, Ruprecht RM, Crotty S, Irvine DJ. Sci Immunol. 2021, 6:eabf1152. PMID: 34860581
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Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and pre-pandemic coronaviruses.
Feldman J, Bals J, Altomare CG, Denis KS, Lam EC, Hauser BM, Ronsard L, Sangesland M, Moreno TB, Okonkwo V, Hartojo N, Balazs AB, Lingwood D*, Schmidt AG*. Sci Immunol. 2021, eabl5842. PMID: 34648356 *co-corresponding
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Engineering an antibody V gene-selective vaccine.
Ronsard L, Yousif AS, Peabody J, Okonkwo V, Devant P, Mogus AT, Barnes RM, Rohrer D, Lonberg N, Peabody D, Chackerian B, and Lingwood D. Front Immunol. 2021, 12:738471. PMID: 34566992
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Fecal SARS-CoV-2 RNA is associated with decreased COVID-19 survival.
Das Adhikari U, Eng G, Farcasanu M, Avena LE, Choudhary MC, Triant VA, Flagg M, Schiff AE, Gomez I, Froehle LM, Diefenbach TJ, Ronsard L, Lingwood D, Lee GC, Rabi SA, Erstad D, Velmahos G, Li JZ, Hodin R, Stone JR, Honko AN, Griffiths A, Yilmaz O, Kwon DS. Clin Infect Dis. 2021, ciab623. PMID: 34245255
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The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells.
Yousif AS, Ronsard R, Shah P, Omatsu T, Sangesland M, Bracamonte Moreno T, Lam EC, Vrbanac VD, Balazs AB, Reinecker H-C, and Lingwood D. Immunity. 2021, 54:235-246. PMID: 33357409
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Antibody Focusing to Conserved Sites of Vulnerability: The Immunological Pathways for ‘Universal’ Influenza Vaccines.
Sangesland M, Lingwood D. Vaccines. 2021. 9:125. PMID: 33562627
2020
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COVID-19-neutralizing antibodies predict disease severity and survival.
Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt AG, Iafrate AJ, Balazs AB. Cell. 2020. 148:476-488. PIMD:33412089
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A single human VH gene allows for a broad-spectrum antibody response targeting bacterial lipopolysaccharides in the blood.
Sangesland M, Yousif A, Ronsard L, Kazer SW, Zhu AL, Gatter GJ, Hayward MR, Barnes R, Quirindongo-Crespo M, Rohrer D, Lonberg N, Kwon D, Shalek AK and Lingwood D. Cell Reports. 2020, 32:108065. PMID: 32846123
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Defining and manipulating B cell immunodominance hierarchies to elicit broadly neutralizing antibody responses against influenza virus.
Amitai A, Sangesland M, Barnes R, Rohrer D, Lonberg N, Lingwood D*, and Chakraborty A*. Cell Systems. 2020, 11:1-16. PMID: 33031741 *co-corresponding
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Loss of Bcl-6-Expressing T Follicular Helper Cells and Germinal Centers in COVID-19.
Kaneko N, Kuo HH, Boucau J, Farmer JR, Allard-Chamard H, Mahajan VS, Piechocka-Trocha A, Lefteri K, Osborn M, Bals J, Bartsch YC, Bonheur N, Caradonna TM, Chevalier J, Chowdhury F, Diefenbach TJ, Einkauf K, Fallon J, Feldman J, Finn KK, Garcia-Broncano P, Hartana CA, Hauser BM, Jiang C, Kaplonek P, Karpell M, Koscher EC, Lian X, Liu H, Liu J, Ly NL, Michell AR, Rassadkina Y, Seiger K, Sessa L, Shin S, Singh N, Sun W, Sun X, Ticheli HJ, Waring MT, Zhu AL, Alter G, Li JZ, Lingwood D, Schmidt AG, Lichterfeld M, Walker BD, Yu XG, Padera RF Jr, Pillai S; Massachusetts Consortium on Pathogen Readiness Specimen Working Group. Cell. 2020, 183:143-157. PMID: 32877699
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SARS-CoV-2receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected inspecific cell subsets across tissues.
Ziegler, C.G.K., Allon, S.J., Nyquist, S.K., Mbano, I.M., Miao, V.N., Tzouanas,C.N., Cao, Y., Yousif, A.S., Bals, J., Hauser, B.M., Feldman, J., Muus, C., Wadsworth II., M.H., Kazer,S.W., Hughes, T.K., Doran, B., Gatter, G.J., Vukovic, M., Taliaferro, F., Mead, B.E., Guo, Z., Wang, J.P.,Gras, D., Plaisant, M., Ansari, M., Angelidis, I., Adler, H., Sucre, J.M.S., Taylor, C.J., Lin, B., Waghray,A., Mitsialis, V., Dwyer, D.F., Buchheit, K.M., Boyce, J.A., Barrett, N.A., Laidlaw, T.M., Carroll, S.L.,Colonna, L., Tkachev, V., Peterson, C.W., Yu, A., Zheng, H.B., Gideon, H.P., Winchell, C.G., Lin, P.L.,Bingle, C.D., Snapper, S.B., Kropski, J.A., Theis, F.J., Schiller, H.B., Zaragosi, L.-E., Barbry, P., Leslie, A., Kiem, H.-P., Flynn, J.L., Fortune, S.M., Berger, B., Finberg, R.W., Kean, L.S., Garber, M., Schmidt,A.G., Lingwood, D., Shalek, A.K., Ordovas-Montanes, J., HCA Lung Biological Network, Cell. 2020, 28:1016-1035. PMID: 32413319
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Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection.
Li B, Clohisey SM, Chia BS, Wang B, Cui A, Eisenhaure T, Schweitzer LD, Hoover P, Parkinson NJ, Nachshon A, Smith N, Regan T, Farr D, Gutmann MU, Bukhari SI, Law A, Sangesland M, Gat-Viks I, Digard P, Vasudevan S, Lingwood D, Dockrell DH, Doench JG, Baillie JK, Hacohen N. Nat Commun. 2020, 11:164. PMID:28827449
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Spontaneous glycan reattachment following N-glycanase treatment of influenza and HIV vaccine antigens.
Keating CL, Kuhn E, Bals J, Cocco AR, Yousif AS, Matysiak C, Sangesland M, Ronsard L, Smoot M, Bracamonte Moreno T, Okonkwo V, Setliff I, Georgiev I, Balazs AB, Carr SA, Lingwood D. J Proteome Res. 2020, 19:733-743. PMID:319136
2019
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High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity.
Ian Setliff, Andrea R. Shiakolas, Kelsey A. Pilewski, Amyn A. Murji, Rutendo E. Mapengo, Katarzyna Janowska, Simone Richardson, Charissa Oosthuysen, Nagarajan Raju, Larance Ronsard, Masaru Kanekiyo, Juliana S. Qin, Kevin J. Kramer, Allison R. Greenplate, Wyatt J. McDonnell, Barney S. Graham, Mark Connors, Daniel Lingwood, Priyamvada Acharya, Lynn Morris, and Ivelin S. Georgiev. Cell. 2019, 179:1636-1646. PMID:28827449
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Germline-Encoded Affinity for cognate antigen enables vaccine amplification of a human broadly neutralizing response against influenza virus.
Sangesland M, Ronsard L, Kazer SW, Bals J, Boyoglu-Barnum S, Yousif AS, Barnes R, Feldman J, Quirindongo-Crespo M, McTamney PM, Rohrer D, Lonberg N, Chackerian B, Graham BS, Kanekiyo M, Shalek AK, Lingwood D. Immunity. 2019, 51:735-749. PMID:28827449
2018
2017
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Correction: Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies.
Nandin IS, Fong C, Deantonio C, Torreno-Pina JA, Pecetta S, Maldonado P, Gasparrini F, Ordovas-Montanes J, Kazer SW, Kjaer S, Borley DW, Nair U, Coleman JA, Lingwood D, Shalek AK, Meffre E, Poignard P, Burton DR, Batista FD. J Exp Med. 2017, 214:2811. PMID: 28827449
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Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies.
Sanjuan Nandin I, Fong C, Deantonio C, Torreno-Pina JA, Pecetta S, Maldonado P, Gasparrini F, Ordovas-Montanes J, Kazer SW, Kjaer S, Borley DW, Nair U, Coleman JA, Lingwood D, Shalek AK, Meffre E, Poignard P, Burton DR, Batista FD. J Exp Med. 2017, 214:2471-2490. PMID: 28739603
2016
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Reconstituted B cell receptor signaling reveals carbohydrate-dependent mode of activation.
Villar RF, Patel J, Weaver GC, Kanekiyo M, Wheatley AK, Yassine HM, Costello CE, Chandler KB, McTamney PM, Nabel GJ, McDermott AB, Mascola JR, Carr SA, Lingwood D. Sci Rep. 2016, 6:36298. PMID: 27796362
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In vitro reconstitution of B cell receptor-antigen interactions to evaluate potential vaccine candidates.
Weaver GC, Villar RF, Kanekiyo M, Nabel GJ, Mascola JR, Lingwood D. Nat Protoc. 2016, 11:193-213. PMID: 26741406
2015
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Hemagglutinin-stem nanoparticles generate heterosubtypic influenza protection.
Yassine HM, Boyington JC, McTamney PM, Wei CJ, Kanekiyo M, Kong WP, Gallagher JR, Wang L, Zhang Y, Joyce MG, Lingwood D, Moin SM, Andersen H, Okuno Y, Rao SS, Harris AK, Kwong PD, Mascola JR, Nabel GJ, Graham BS. Nat Med. 2015, 21:1065-1070. PMID: 2630169
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H5N1 Vaccine-Elicited Memory B Cells Are Genetically Constrained by the IGHV Locus in the Recognition of a Neutralizing Epitope in the Hemagglutinin Stem.
Wheatley AK, Whittle JR, Lingwood D, Kanekiyo M, Yassine HM, Ma SS, Narpala SR, Prabhakaran MS, Matus-Nicodemos RA, Bailer RT, Nabel GJ, Graham BS, Ledgerwood JE, Koup RA, McDermott AB. J Immunol. 2015, 195:602-610. PMID: 26078272
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Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors.
Zhou T, Lynch RM, Chen L, Acharya P, Wu X, Doria-Rose NA, Joyce MG, Lingwood D, Soto C, Bailer RT, Ernandes MJ, Kong R, Longo NS, Louder MK, McKee K, O’Dell S, Schmidt SD, Tran L, Yang Z, Druz A, Luongo TS, Moquin S, Srivatsan S, Yang Y, Zhang B, Zheng A, Pancera M, Kirys T, Georgiev IS, Gindin T, Peng HP, Yang AS, Mullikin JC, Gray MD, Stamatatos L, Burton DR, Koff WC, Cohen MS, Haynes BF, Casazza JP, Connors M, Corti D, Lanzavecchia A, Sattentau QJ, Weiss RA, West AP, Bjorkman PJ, Scheid JF, Nussenzweig MC, Shapiro L, Mascola JR, Kwong PD. Cell. 2015, 161:1280-1292. PMID: 26004070
2014
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Lipocalin 2 as a membrane-reorganizing agent.
Lingwood D. Sci Signal. 2014, 7:19. PMID: 25118326
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Flow cytometry reveals that H5N1 vaccination elicits cross-reactive stem-directed antibodies from multiple Ig heavy-chain lineages.
Whittle JR, Wheatley AK, Wu L, Lingwood D, Kanekiyo M, Ma SS, Narpala SR, Yassine HM, Frank GM, Yewdell JW, Ledgerwood JE, Wei CJ, McDermott AB, Graham BS, Koup RA, Nabel GJ. J Virol. 2014, 88:4047-4057. PMID: 24501410
2012
2011
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Molecular convergence of bacterial and eukaryotic surface order.
Kaiser HJ, Surma MA, Mayer F, Levental I, Grzybek M, Klemm RW, Da Cruz S, Meisinger C, Müller V, Simons K, Lingwood D. J Biol Chem. 2011, 286:40631-40637. PMID: 21965671
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Lateral sorting in model membranes by cholesterol-mediated hydrophobic matching.
Kaiser HJ, Orlowski A, Róg T, Nyholm TK, Chai W, Feizi T, Lingwood D, Vattulainen I, Simons K. Proc Natl Acad Sci U S A. 2011, 108:16628-16633. PMID: 21930944
2010
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Palmitoylation regulates raft affinity for the majority of integral raft proteins.
Levental I, Lingwood D, Grzybek M, Coskun U, Simons K. Proc Natl Acad Sci U S A. 2010, 107:22050-22054. PMID: 21131568
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Lipid rafts as a membrane-organizing principle.
Lingwood D, Simons K. Science. 2010, 327:46-50. PMID: 20044567
2009
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Morphological homeostasis by autophagy.
Lingwood D, Schuck S, Ferguson C, Gerl M, Simons K. Autophagy. 2009, 5:1039-40. PMID: 19587538
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Lipid rafts as functional heterogeneity in cell membranes.
Lingwood D, Kaiser HJ, Levental I, Simons K. Biochem Soc Trans. 2009, 37:955-960. PMID: 19754431
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Order of lipid phases in model and plasma membranes.
Kaiser HJ, Lingwood D, Levental I, Sampaio JL, Kalvodova L, Rajendran L, Simons K. Proc Natl Acad Sci U S A. 2009, 106:16645-16650. PMID: 19805351
2008
2007
2006
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Decoupling the Na+-K+-ATPase in vivo: a possible new role in the gills of freshwater fishes.
Lingwood D, Harauz G, Ballantyne JS. Comp Biochem Physiol A Mol Integr Physiol. 2006, 144:451-457. PMID: 16730202
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Alkaline phosphatase-immunoglobulin conjugate binds to lipids in vitro, independent of antibody selectivity.
Lingwood D, Ballantyne JS. J Immunol Methods. 2006, 311:174-177. PMID: 16524587